CTCF Rabbit Polyclonal Antibody

CAT#: TA319396

Rabbit polyclonal anti-CTCF antibody

Product Images

Western Blot of Rabbit Anti-CTCF antibody. Lane 1: H929 nuclear cell lysates. Load: 30 ug per lane. Primary antibody: CTCF antibody at 1.2 ug/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 3% BSA overnight at 4°C. Predicted/Observed size: 82.8 kDa or 150 kDa for CTCF. Other band (s): CTCF splice variants.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:10,000-1:50,000, WB: 1:500-1:2:000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CCCTC-binding factor
• Database Link:
  NP_006556
  Entrez Gene 13018 MouseEntrez Gene 10664 Human
  UniProt ID P49711
• Synonyms: MRD21
• Note: Transcriptional repressor CTCF (also known as CCCTC-binding factor) is a transcription factor encoded by the CTCF gene. The CTCF, and the closely related Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved zinc finger proteins implicated in diverse regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. Expression of BORIS is restricted to spermatocytes and is mutually exclusive of CTCF expression. CTCF is ubiquitously expressed in higher eukaryotes and contains a highly conserved and eleven zinc finger central DNA-binding domain, having very high homology between mouse, chicken, and human and is embedded within slightly divergent N and C termini. CTFC plays a critical role in the epigenetic regulation and chromatin remodeling. CTCF has been reported to bind to a variety of DNA target sites that perform distinct functions, including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting.

Reference Data

• Protein Families: Transcription Factors