Adgre5 Rabbit Polyclonal Antibody

CAT#: TA319372

Rabbit polyclonal anti-CD97 antibody

Product Images

WB using anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). The formation of the CD97 complex is currently under investigation. A 1:1,000 dilution of the primary antibody was used.

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  Immunofluorescence Microscopy using Protein A purified anti-CD97 antibody shows staining of Fc-CD97- (5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:2, 500 dilution of the primary antibody was used. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000, IF: 1:1,000 - 1:5,000
• Reactivities: Human, Chimpanzee
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adhesion G protein-coupled receptor E5
• Database Link:
  NP_001156501
  Entrez Gene 976 Human
  UniProt ID Q9Z0M6
• Note: This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including