Ankrd26 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:3,000 ELISA: 1:20,000 - 1:80,000 |
| Reactivity | Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of mouse Ankrd26 protein. |
| Specificity | This affinity-purified antibody is directed against mouse Ankrd26 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Ankrd26 proteins from mouse (100%), rat (93%), bovine (81%) and human (68%). Reactivity against homologues from other sources is not known. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.48 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | ankyrin repeat domain 26 |
| Database Link | |
| Background | This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. POTE gene is expressed in prostate and breast cancer and a potential candidate for immuno-therapy. It is also expressed in undifferentiated embryonic stem (ES) cells. ANKRD-26 is the ancestral gene of POTE and has a homologue in mouse. Mouse Ankrd26 is also selectively expressed in ES cells and various cancers but not in other adult tissues. The encoded protein of Ankrd26 contains ankyrin repeats and coiled coil domain of spectrin suggestive of a signaling molecule. |
| Synonyms | rabbit anti-Ankrd26 Antibody, ankrd 26, ankrd-26, Ankyrin repeat domain 26 antibody, Ankyrin repeat domain containing protein 26 antibody, bA145E8.1 antibody, KIAA1074 antibody |
| Note | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to Ankrd26 by western blotting in the appropriate mouse tissue. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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