RFX5 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ChIP, ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:1,000 CHiP: User Optimized ELISA: 1:5,000 - 1:25,000 Gel Shift: 0.5 µL to 1.0 µL |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Specificity | RFXF5 antibody was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | regulatory factor X5 |
| Database Link | |
| Background | Anti-RFX5 is specific for the RFX5 protein. RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. RFX5 has been shown to interact with CIITA. |
| Synonyms | rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody |
| Note | Anti RFX5 antibody has been assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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