IL32 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein. |
| Specificity | This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-32A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: None |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Gene Name | interleukin 32 |
| Database Link | |
| Background | IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32α is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFα and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Anti-IL-32A antibody is ideal for investigators involved in Cancer and Immunology research. |
| Synonyms | rabbit anti-IL-32A antibody, rabbit anti-interleukin-32A antibody, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32, IL-32 isoform 4 |
| Note | This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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