AURKB Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IF, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:250 - 1:2,000 IHC: User Optimized IF: User Optimized ELISA: 1:10,000 - 1:30,000 |
| Reactivity | Human, Monkey |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein. |
| Specificity | Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 0.87 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store Phospho Specific Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | aurora kinase B |
| Database Link | |
| Background | Aurora Kinase B (Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Anti-AUROA B pT232 Antibody is useful for researchers interested in gene expression, DNA damage, cytokinesis, and transcription activities. |
| Synonyms | rabbit anti-Aurora B pT232 Antibody, Phospho Aurora B, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody |
| Note | Phospho pT232 Aurora B antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. See below for specific protocol. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. HeLa cell lysate can be used as a positive control. |
| Reference Data | |
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Citations
Exploring kinase substrate selectivity and novel mechanisms of kinase regulation
Babisz
Thesis (2025)
Babisz
Thesis (2025)
AI Summary: OriGene’s Aurora B pT232 antibody (TA319253) was used in this study to detect phosphorylated Aurora B kinase, aiding in the exploration of kinase substrate selectivity and regulatory mechanisms.
Snippet: Aurora B (AIM1) BD Biosciences/ 611082 Mouse 1:1000 Aurora B pT232 Origene/ TA319253 Rabbit 1:2000 GFP Crick (In-house) Mouse 1:1000 Thiophosphate Ester Abcam/ AB92570 Rabbit 1:5000 β -Tubulin Proteintech/ 15115S Rabbit 1:3000
The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition
Kelly, Martini, Brownlow et al
Nat Commun (2020) 11 (1), 1396 DOI: 10.1038/s41467-020-15163-6
Kelly, Martini, Brownlow et al
Nat Commun (2020) 11 (1), 1396 DOI: 10.1038/s41467-020-15163-6
Cited by 14
AI Summary: In this study, the OriGene rabbit anti-Aurora B phosphoThr232 antibody (TA319253) was used to detect phosphorylated Aurora B, enabling the authors to investigate the role of Aurora B phosphorylation in protecting against chromosome non-disjunction during mitosis.
Snippet: … The following primary antibodies were used: mouse anti-Aurora B (AIM-1,611082, BD, 1:2000), rabbit anti-Aurora B phosphoThr232 (TA319253, Origene, 1:1000), rabbit anti-Aurora B phosphoSer227 (made in-house, 1:1000), mouse anti-alpha tubulin (clone DM1A, T9026, Sigma, 1:10000), mouse anti-TopoIIα (clone KiS1, MAB4197, Millipore 1:5000),;- Page 1. …
PKCɛ switches Aurora B specificity to exit the abscission checkpoint
Pike, Brownlow, Kjaer et al
Nat Commun (2016) 7, 13853 DOI: 10.1038/ncomms13853
Pike, Brownlow, Kjaer et al
Nat Commun (2016) 7, 13853 DOI: 10.1038/ncomms13853
AI Summary: OriGene’s rabbit anti-Aurora B phosphoThr232 antibody (TA325250) was used in this study to detect the phosphorylation status of Aurora B, thereby contributing to the analysis of PKCɛ’s role in modulating Aurora B specificity during abscission checkpoint exit.
Snippet: The following primary antibodies were used in these assays are as follows: mouse anti-Aurora B (AIM-1,611082, BD, 1:500);rabbit anti-Aurora B phosphoThr232 (TA325250, Origene, 1:300);rabbit anti-Aurora B phosphoSer227 (made in-house, 1:100);mouse anti- ... Cite SaveSaving
Showing 1-3 of 3 papers.
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