Camk2a Rabbit Polyclonal Antibody

CAT#: TA319219

Rabbit polyclonal CaM Kinase II antibody

Product Images

WB using Anti-CaM Kinase II antibody shows detection of a band ~54 kDa corresponding to human alpha CaM Kinase II (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). The primary antibody diluted to 1:1, 500 for 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:4,000 - 1:20,000, WB: 1:1,000 - 1:3,000
• Reactivities: Human, Mouse, Rat, Dog, Orang-Utan
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 6-23 of Human CaM Kinase II protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: calcium/calmodulin-dependent protein kinase II alpha
• Database Link:
  NP_037052
  Entrez Gene 815 HumanEntrez Gene 12322 MouseEntrez Gene 612941 DogEntrez Gene 25400 Rat
  UniProt ID P11275
• Synonyms: CAMKA; CaMKIINalpha; KIAA0968
• Note: CaM Kinase II (also known as CAMK2 and calcium/calmodulin-dependent protein kinase type II alpha chain) is a prominent kinase in the central nervous system that may function in the long-term potentiation of neurotransmitter release. CaM Kinase II autophosphorylates itself at Thr-286 which allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CaM Kinase II is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. This kinase is expressed in brain tissue. Alternative splicing occurs for this gene product.