BAG3 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:40000 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | The antiserum was produced against synthesized peptide derived from internal of human BAG3. |
| Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | lot specific |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | BCL2 associated athanogene 3 |
| Database Link | |
| Synonyms | BAG-3; BIS; CAIR-1; MFM6 |
| Note | BAG3 antibody detects endogenous levels of total BAG3 protein. |
| Reference Data | |
| Protein Categories | Cardiovascular diseases, Intracellular Proteins |
| Protein Families | Druggable Genome |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
The Lifeact-EGFP mouse is a translationally controlled fluorescent reporter of T cell activation
Galeano Niño, Tay, Tearle et al
J. Cell. Sci. (2020)
Galeano Niño, Tay, Tearle et al
J. Cell. Sci. (2020)
Cited by 13
AI Summary: OriGene's polyclonal BAG3 antibody was used in this study to probe for BAG3 protein expression in Western blot analyses, contributing to the investigation of translational regulation in T cell activation.
Snippet: Membranes were probed with primary antibodies against GFP (Abcam, polyclonal), BAG3 (Origene, polyclonal) and Tubulin (Abcam, YL1/2) at 1:1000, 1:1000 and 1:5000 dilutions, respectively. Followed by incubation with secondary antibodies anti-rabbit IgG (Amersham, 1:2000) and goat anti-rat (Thermo Fisher Scientific, 1:10,000). …
Withaferin A Induces Cell Death Selectively in Androgen-Independent Prostate Cancer Cells but Not in Normal Fibroblast Cells
Nishikawa, Okuzaki, Fukushima et al
PLoS ONE (2015) 10 (7), e0134137 DOI: 10.1371/journal.pone.0134137
Nishikawa, Okuzaki, Fukushima et al
PLoS ONE (2015) 10 (7), e0134137 DOI: 10.1371/journal.pone.0134137
Cited by 70
AI Summary: OriGene siRNAs targeting c-Fos, PARP, and FLIP were used in this study to assess the roles of these proteins in Withaferin A-induced cell death in prostate cancer cells, helping to demonstrate the molecular mechanisms underlying selective cytotoxicity.
Snippet: NT, non-treated. (C) Western blot analysis to detect c-Fos, PARP, FLIP and GAPDH in PC-3 cells at 12 h after 4 μM WA treatment in the presence (+) or absence (-) of three different siRNAs (X–Z) from OriGene. (D) Viability of PC-3 cells after siFos treatment. ... Cite SaveSaving
Showing 1-2 of 2 papers.
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