CEP135 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | WB |
|---|---|
| Recommended Dilution | WB: 1:500~1:3000, ELISA: 1:40000 |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | The antiserum was produced against synthesized peptide derived from C-terminal of human CEP135. |
| Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | lot specific |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | centrosomal protein 135 |
| Database Link | |
| Synonyms | CEP4; KIAA0635; MCPH8 |
| Note | CEP135 antibody detects endogenous levels of total CEP135 protein. |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Hsp72 and Nek6 cooperate to cluster amplified centrosomes in cancer cells
Sampson, O'Regan, Dyer et al
Cancer Res. (2017)
Sampson, O'Regan, Dyer et al
Cancer Res. (2017)
AI Summary: In this study, the OriGene anti-CEP135 antibody was used to detect and analyze centrosome components, helping to elucidate the mechanisms by which Hsp72 and Nek6 cooperate to cluster amplified centrosomes in cancer cells.
Snippet: Hepes, pH7.4, 10 mM EGTA, 2 mM MgCl2, and 1% Triton X-100) prior to fixation. Primary antibodies were against α-tubulin (0.3 µg/ml;Sigma), γ-tubulin (0.5 µg/ml;Sigma), centrin-2 (N- 17) (0.4 µg/ml;Santa Cruz Biotechnology), CEP135 (2 µg/ml;OriGene), pericentrin ... …
Showing 1-1 of 1 papers.
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