PCB (PC) Rabbit Polyclonal Antibody

CAT#: TA309794

Rabbit Polyclonal Pyruvate Carboxylase Antibody

Product Images

Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody TA309794 - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (TA309794) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody TA309794 - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse adipose tissue using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a specific staining of the fat cells in the tested section.

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  Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody TA309794 - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Pyruvate Carboxylase at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody TA309794 - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a diffused cytoplasmic staining in all the tubules and a subset of cells in glomerular cells.

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  Western Blot: Pyruvate Carboxylase Antibody TA309794 - Analysis of Pyruvate Carboxylase in A. Human liver extracts and B. Mouse liver extracts

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Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: Knockdown Validated, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:50-1:100, Immunohistochemistry-Paraffin: 1:200, Western Blot: 1:1000
• Reactivities: Bovine, Human, Mouse, Porcine, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A genomic peptide made to an internal region of the human Pyruvate Carboxylase protein (within residues 930-1050). [Swiss-Prot P11498]
• Formulation: PBS, 0.1% BSA, 50% glycerol and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: pyruvate carboxylase
• Database Link:
  NP_000911
  Entrez Gene 25104 RatEntrez Gene 18517 MouseEntrez Gene 5091 Human
  UniProt ID P11498
• Synonyms: PCB

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pyruvate metabolism