DDIT3 Mouse Monoclonal Antibody [Clone ID: 9C8]

CAT#: TA309621

Mouse Monoclonal CHOP/GADD153 Antibody (9C8)

Product Images

Simple Western: GADD153/CHOP Antibody (9C8) TA309621 - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) TA309621 - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.

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  Flow Cytometry: GADD153/CHOP Antibody (9C8) TA309621 - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody (9C8) TA309621 (blue) and a matched isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

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  Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) TA309621 - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.

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  Western Blot: GADD153/CHOP Antibody (9C8) TA309621 - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).

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  Western Blot: GADD153/CHOP Antibody (9C8) TA309621 - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using TA309621. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours.

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Specifications

Product Data

• Clone Name: 9C8
• Applications: ChIP, ELISA, FC, ICC/IF, IHC, IP, Simple Western, WB
• Recommended Dilution: Flow Cytometry, Immunoprecipitation: 1:10 - 1:500, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot, Immunohistochemistry-Paraffin: 1:100, Gel Super Shift Assays, Simple Western: 1:250, Chromatin Immunoprecipitation (ChIP), ELISA, Knockdown Validated
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: Full length mouse CHOP/GADD153 [Swiss-Prot# P35639]
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DNA damage inducible transcript 3
• Database Link:
  NP_004074
  Entrez Gene 13198 MouseEntrez Gene 1649 Human
  UniProt ID P35638
• Synonyms: CEBPZ; CHOP; CHOP-10; CHOP10; GADD153

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: MAPK signaling pathway