ABCA1 Rabbit Polyclonal Antibody

CAT#: TA309555

Rabbit Polyclonal ABCA1 Antibody

Product Images

Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.

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  ABCA1 antibody was tested at 1: 400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

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  Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate.

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  Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.

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  Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # TA309555) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

• HepG2 cells were grown to 60% confluency, serum starved for 24 hours, and then treated with 1uM TO9 for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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Specifications

Product Data

• Applications: Block/Neutralize, ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
• Recommended Dilution: Simple Western: 1:50, Gel Supershift Assay, Immunohistochemistry-Paraffin: 1:200, Immunohistochemistry-Frozen, Immunohistochemistry: 1:200, Western Blot: 1:500, Dual RNAscope ISH-IHC, Flow Cytometry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:100, Immunoprecipitation: 1:10-1:500, Gel Super Shift Assays, Immunoblotting, Block/Neutralize, PCR, Chromatin Immunoprecipitation, ELISA, Knockdown Validated, Knockout Validated, Chromatin Immunoprecipitation (ChIP)
• Reactivities: Human, Mouse, Rat, Hamster
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.
• Formulation: Tris-citrate pH 7-8 and 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATP binding cassette subfamily A member 1
• Database Link:
  NP_005493
  Entrez Gene 11303 MouseEntrez Gene 313210 RatEntrez Gene 19 Human
  UniProt ID O95477
• Synonyms: ABC-1; ABC1; CERP; HDLDT1; TGD

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters