Map2k1 Rabbit Polyclonal Antibody

CAT#: TA309167

Rabbit Anti-MEK1 (Thr292) Antibody (Phospho-Specific)

Product Images

Western blot of recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human, Bovine, Rat, Dog
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 conjugated to KLH
• Formulation: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: mitogen-activated protein kinase kinase 1
• Database Link:
  NP_032953
  Entrez Gene 5604 HumanEntrez Gene 170851 RatEntrez Gene 478347 Dog
  UniProt ID P31938
• Background: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq]
• Synonyms: MAPKK1; MEK1; MKK1; PRKMK1