Marcks Rabbit Polyclonal Antibody

CAT#: TA309164

Rabbit Anti-MARCKS (Ser152,156) Antibody (Phospho-Specific)

Product Images

Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152, 156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser152, 156 antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH
• Formulation: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87 kDa
• Gene Name: myristoylated alanine rich protein kinase C substrate
• Database Link:
  NP_001258019
  Entrez Gene 25603 Rat
• Background: This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]
• Synonyms: 80K-L; FLJ14368; FLJ90045; MACS; MRACKS; phosphomyristin; PKCSL; PRKCSL