SUCLA2 Rabbit Polyclonal Antibody

CAT#: TA308975

Rabbit Polyclonal antibody to SUCLA2 (succinate-CoA ligase, ADP-forming, beta subunit)

Product Images

Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. TA308975 diluted at 1:20000.

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  Sample (30 ug of whole cell lysate). A: Hep G2. B: Molt-4. 10% SDS PAGE. TA308975 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SUCLA2 (TA308975) antibody at 1:500 dilution.

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  Immunofluorescence analysis of methanol-fixed A431, using SUCLA2 (TA308975) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:20000
• Reactivities: Human, Mouse (Predicted: Rat, Xenopus, Zebrafish, Bovine, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 138 and 416 of SUCLA2 (Uniprot ID#Q9P2R7)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: succinate-CoA ligase ADP-forming beta subunit
• Database Link:
  NP_003841
  Entrez Gene 20916 MouseEntrez Gene 361071 RatEntrez Gene 8803 Human
  UniProt ID Q9P2R7
• Background: Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq]
• Synonyms: A-BETA; MTDPS5; SCS-betaA
• Note: Seq homology of immunogen across species: Rat (88%), Xenopus laevis (85%), Zebrafish (81%), Bovine (92%), Xenopus tropicalis (83%)

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Propanoate metabolism