MCM3 Rabbit Polyclonal Antibody

CAT#: TA308761

Rabbit Polyclonal antibody to MCM3 (minichromosome maintenance complex component 3)

Product Images

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. TA308761 diluted at 1:10000.

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  Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MCM3 (TA308761) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3 (TA308761) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
• Reactivities: Human (Predicted: Rhesus Monkey)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 42 and 302 of MCM3 (Uniprot ID#P25205)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91 kDa
• Gene Name: minichromosome maintenance complex component 3
• Database Link:
  NP_002379
  Entrez Gene 4172 Human
  UniProt ID P25205
• Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]
• Synonyms: HCC5; P1-MCM3; P1.h; RLFB
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication