VAV1 Rabbit Polyclonal Antibody

CAT#: TA308739

Rabbit polyclonal antibody to VAV1 (vav 1 guanine nucleotide exchange factor)

Product Images

Sample (30 ug of whole cell lysate). A:MOLT4. B:Raji. 7.5% SDS PAGE. TA308739 diluted at 1:500

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  VAV1 antibody detects VAV1 protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. 7.5 % SDS-PAGE. VAV1 antibody (TA308739) dilution: 1:1000

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  Immunohistochemical analysis of paraffin-embedded serous OVCA, using VAV1 (TA308739) antibody at 1:100 dilution.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Rat, Bovine)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 83 and 568 of VAV1 (Uniprot ID#P15498)
• Formulation: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 98 kDa
• Gene Name: vav guanine nucleotide exchange factor 1
• Database Link:
  NP_005419
  Entrez Gene 22324 MouseEntrez Gene 25156 RatEntrez Gene 7409 Human
  UniProt ID P15498
• Background: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]
• Synonyms: VAV
• Note: Seq homology of immunogen across species: Mouse (92%), Rat (92%), Bovine (96%)

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway