VPS35 Rabbit Polyclonal Antibody

CAT#: TA308721

Rabbit Polyclonal antibody to VPS35 (vacuolar protein sorting 35 homolog (S. cerevisiae))

Product Images

VPS35 antibody detects VPS35 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. VPS35 antibody (TA308721) dilution: 1:500

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  Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308721 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A:H1299. B:HeLa S3. C:Hep G2. D:MOLT4. E:Raji. 7.5% SDS PAGE. TA308721 diluted at 1:500

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  VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term (TA308721) dilution: 1:250.

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  Immunofluorescence analysis of methanol-fixed HCT116, using VPS35 (TA308721) antibody at 1:500 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Zebrafish, Chicken, Bovine)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID#Q96QK1)
• Formulation: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 92 kDa
• Gene Name: VPS35 retromer complex component
• Database Link:
  NP_060676
  Entrez Gene 65114 MouseEntrez Gene 55737 Human
  UniProt ID Q96QK1
• Background: This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]
• Synonyms: MEM3; PARK17
• Note: Seq homology of immunogen across species: Zebrafish (86%), Chicken (93%), Bovine (100%)