FASTK Rabbit Polyclonal Antibody

CAT#: TA308720

Rabbit Polyclonal antibody to FAST (Fas-activated serine/threonine kinase)

Product Images

FAST antibody detects FASTK protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lys

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  Sample (30 ug of whole cell lysate). A:NIH-3T3. 7.5% SDS PAGE. TA308720 diluted at 1:1000.

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  FAST antibody detects FASTK protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5 % SDS-PAGE. FAST antibody (TA308720) dilution: 1:1000

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  Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST (TA308720) antibody at 1:100 dilution.

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  Immunofluorescence analysis of methanol-fixed HeLa, using FAST (TA308720) antibody at 1:200 dilution.

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  FAST antibody detects FAST protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FAST protein stained by FAST antibody (TA308720) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 346 and 549 of FAST (Uniprot ID#Q14296)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Affinity purified by Protein A.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61 kDa
• Gene Name: Fas activated serine/threonine kinase
• Database Link:
  NP_006703
  Entrez Gene 66587 MouseEntrez Gene 10922 Human
  UniProt ID Q14296
• Background: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq]
• Synonyms: FAST

Reference Data

• Protein Families: Druggable Genome, Protein Kinase