MCM7 Rabbit Polyclonal Antibody

CAT#: TA308526

Rabbit polyclonal antibody to MCM7 (minichromosome maintenance complex component 7)

Product Images

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. TA308526 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7 (TA308526) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM7 (TA308526) antibody at 1:200 dilution.

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  MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 anti

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  Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Rat, Zebrafish, Bovine)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 190 and 481 of MCM7 (Uniprot ID#P33993)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61 kDa
• Gene Name: minichromosome maintenance complex component 7
• Database Link:
  NP_877577
  Entrez Gene 17220 MouseEntrez Gene 288532 RatEntrez Gene 4176 Human
  UniProt ID P33993
• Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
• Synonyms: CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
• Note: Seq homology of immunogen across species: Mouse (92%), Rat (93%), Zebrafish (85%), Bovine (98%)

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle, DNA replication