STIP1 Rabbit Polyclonal Antibody

CAT#: TA308465

Rabbit Polyclonal antibody to STIP1 (stress-induced-phosphoprotein 1)

Product Images

STIP1 antibody detects STIP1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole

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  Sample (30 ug of whole cell lysate). A:Hep G2. B:MOLT4. 7.5% SDS PAGE. TA308465 diluted at 1:5000

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  Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using STIP1 (TA308465) antibody at 1:100 dilution.

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  Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1 (TA308465) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 300 of STIP1 (Uniprot ID#P31948)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63 kDa
• Gene Name: stress induced phosphoprotein 1
• Database Link:
  NP_006810
  Entrez Gene 20867 MouseEntrez Gene 10963 Human
  UniProt ID P31948
• Background: STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]). [supplied by OMIM]
• Synonyms: HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Prion diseases