VPS11 Rabbit Polyclonal Antibody

CAT#: TA308196

Rabbit polyclonal antibody to VPS11 (vacuolar protein sorting 11 homolog (S. cerevisiae))

Product Images

Sample (30 ug of whole cell lysate). A:293T. B:Hep G2. 7.5% SDS PAGE. TA308196 diluted at 1:500

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  VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. VPS11 antibody (TA308196) dilution: 1:500.

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  VPS11 antibody detects VPS11 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20â 100% MeOH for 5 min. Green: VPS11 protein stained by VPS11 antibody (TA308196) diluted at 1:500. Blue: Hoechst 33343 staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Rat, Zebrafish, Bovine, X. tropicalis, Medaka fish)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 300 and 392 of VPS11 (Uniprot ID#Q9H270)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 108 kDa
• Gene Name: VPS11, CORVET/HOPS core subunit
• Database Link:
  NP_068375
  Entrez Gene 71732 MouseEntrez Gene 315600 RatEntrez Gene 55823 Human
  UniProt ID Q9H270
• Background: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq]
• Synonyms: END1; hVPS11; PEP5; RNF108
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (100%), Zebrafish (85%), Japanese Medaka (100%), Xenopus Tropicalis (92%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome