CBS Rabbit Polyclonal Antibody

CAT#: TA308071

Rabbit Polyclonal antibody to CBS (cystathionine-beta-synthase)

Product Images

CBS antibody detects CBS protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. 7.5 % SDS-PAGE. CBS antibody (TA308071) dilution: 1:1000

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  Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using CBS (TA308071) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBS (TA308071) antibody at 1:200 dilution.

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  CBS antibody immunoprecipitates CBS protein in IP experiments. IP samples: HepG2 whole cell extract. A. 50 ug HepG2 whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of CBS protein by 4 ug CBS antibody (TA308071). 7.

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Rabbit, Rat, Bovine, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 39 and 251 of CBS (Uniprot ID#P35520)
• Formulation: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61 kDa
• Gene Name: cystathionine-beta-synthase
• Database Link:
  NP_000062
  Entrez Gene 12411 MouseEntrez Gene 24250 RatEntrez Gene 875 Human
  UniProt ID P35520
• Background: The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]
• Synonyms: HIP4
• Note: Seq homology of immunogen across species: Mouse (94%), Rabbit (95%), Rat (94%), Bovine (94%), Xenopus tropicalis (93%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Selenoamino acid metabolism