MAF1 Rabbit Polyclonal Antibody

CAT#: TA307935

Rabbit polyclonal antibody to MAF1 (MAF1 homolog (S. cerevisiae))

Product Images

Sample (30 ug whole cell lysate). A:A431. B:MOLT4. 12% SDS PAGE. TA307935 diluted at 1:1000

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  MAF1 antibody detects MAF1 protein by Western blot analysis. A. 30 ug GL261 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 12 % SDS-PAGE. MAF1 antibody (TA307935) dilution: 1:1000

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  MAF1 antibody detects MAF1 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 12 % SDS-PAGE. MAF1 antibody (TA307935) dilution: 1:1000

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  MAF1 antibody detects MAF1 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. MAF1 antibody (TA307935) dilution: 1:500.

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  MAF1 antibody detects MAF1 protein at nucleus and cytoplasm on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. MAF1 antibody (TA307935) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 (TA307935) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 201 of MAF1 (Uniprot ID#Q9H063)
• Formulation: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29 kDa
• Gene Name: MAF1 homolog, negative regulator of RNA polymerase III
• Database Link:
  NP_115648
  Entrez Gene 84232 Human
  UniProt ID Q9H063
• Background: This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]
• Synonyms: MGC20332; MGC31779; MGC39758

Reference Data

• Protein Families: Transcription Factors