PLEKHM1 Rabbit Polyclonal Antibody

SKU TA306873

Rabbit Polyclonal PLEKHM1 Antibody

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PLEKHM1 antibody was raised against an 18 amino acid peptide from near the amino terminus of human PLEKHM1.
• Buffer: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: pleckstrin homology and RUN domain containing M1
• Database Link:
  NP_055613
  Q9Y4G2
• Background: PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorbtion when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.
• Synonyms: AP162; B2; OPTB6

Reference Data