THEM4 Rabbit Polyclonal Antibody

CAT#: TA306801

Rabbit Polyclonal THEM4 Antibody

Product Images

Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 ug/ml.

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  Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 ug/ml.

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  Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: THEM4 antibody was raised against an 18 amino acid peptide near the center of human THEM4.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: thioesterase superfamily member 4
• Database Link:
  NP_444283
  Entrez Gene 75778 MouseEntrez Gene 361992 RatEntrez Gene 117145 Human
  UniProt ID Q5T1C6
• Background: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program. The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
• Synonyms: CTMP

Reference Data

• Protein Families: Druggable Genome