SNPH Rabbit Polyclonal Antibody

CAT#: TA306590

Rabbit Polyclonal Syntaphilin Antibody

Product Images

Western blot analysis of Syntaphilin in rat brain tissue lysate with Syntaphilin antibody at (A) 1 and (B) 2 ug/ml.

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  Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 ug/ml.

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  Immunofluorescence of Syntaphilin in Human Brain cells with Syntaphilin antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Syntaphilin antibody was raised against a 16 amino acid peptide from near the center of human Syntaphilin.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: syntaphilin
• Database Link:
  NP_055538
  Entrez Gene 241727 MouseEntrez Gene 296267 RatEntrez Gene 9751 Human
  UniProt ID O15079
• Background: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Multiple isoforms are known to exist.
• Synonyms: bA314N13.5