SLC9A1 Rabbit Polyclonal Antibody

CAT#: TA306492

Rabbit Polyclonal Nhe-1 Antibody

Product Images

Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2ug/ml.

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  Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5ug/ml.

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  Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1. The immunogen is located within the last 50 amino acids of Nhe-1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: solute carrier family 9 member A1
• Database Link:
  NP_003038
  Entrez Gene 20544 MouseEntrez Gene 24782 RatEntrez Gene 6548 Human
  UniProt ID P19634
• Background: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
• Synonyms: APNH; NHE-1; NHE1; PPP1R143

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction, Regulation of actin cytoskeleton