OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

BUB1 Rabbit Polyclonal Antibody

Be the first to review this product
SKU
TA306432
Rabbit Polyclonal Bub1 Antibody
Size:
100 ug
Conjugation:
Unconjugated
  $640.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
LY401381 Transient overexpression lysate of budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) (as WB positive control) 100 ug
$436.00
TP307013 Recombinant protein of human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1), 20 µg
20 ug
$867.00

View other "BUB1 or NM_004336" antibodies

Specifications
Specifications
Product Data
Application IF, WB
Recommended Dilution WB: 2 - 4 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
Reactivity Human, Mouse
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Bub1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human Bub1.
Buffer PBS containing 0.02% sodium azide.
Concentration 1 mg/ml
Purification Affinity chromatography purified via peptide column
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Gene Name BUB1 mitotic checkpoint serine/threonine kinase
Database Link
Background Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Synonyms BUB1A; BUB1L; hBUB1
Reference Data
Protein Categories Cancer: Gastrointestinal, Enzyme: Kinases, Intracellular Proteins
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources

file-alt Citations

TGFBR2 mediated phosphorylation of BUB1 at Ser-318 is required for transforming growth factor-β signaling
Nyati, Gregg, Xu et al
Neoplasia (2020) 22 (4), 163-178 DOI: 10.1016/j.neo.2020.02.001
Cited by 16
AI Summary: OriGene’s BUB1 antibody (#TA306432) was used in this study to detect and analyze BUB1 protein, enabling the investigation of its phosphorylation and interactions in TGF-β signaling.
Snippet: Invitrogen), and Myc-tag (clone 9E10) and His-tag (clone H8) were from Millipore-Sigma. BUB1 (#Ab70372, #Ab9000), Actin-HRP (# ab20272), GAPDH-HRP (#Ab9485) were from Abcam. BUB1 (#TA306432) was from Origene. The HRP-conjugated secondary antibodies were from Jackson ImmunoResearch. Recombinant human TGF-β1 (#100-21B) was obtained from PeproTech, siRNA targeting human BUB1 (5’-GAGUGAUCACGAUUUCUA
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products