Ripk3 Rabbit Polyclonal Antibody

CAT#: TA306042

Rabbit Polyclonal RIP3 Antibody

Product Images

Western blot analysis of RIP3 in HeLa cell lysate with RIP3 antibody at (A) 1 and (B) 2ug/ml.

• 

  Immunohistochemistry of RIP3 in mouse kidney tissue with RIP3 Antibody at 2.5ug/ml.

• 

  Immunohistochemistry of RIP3 in rat kidney tissue with RIP3 antibody at 5ug/ml.

• 

  Immunofluorescence of RIP3 in Rat Kidney cells with RIP3 antibody at 20ug/ml.

• 

  Immunofluorescence of RIP3 in mouse kidney tissue with RIP3 Antibody at 20ug/ml.

Show all 5 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: RIP3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of murine RIP3. The immunogen is located within the last 50 amino acids of RIP3.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: receptor-interacting serine-threonine kinase 3
• Database Link:
  AAF03133
  Entrez Gene 56532 Mouse
  UniProt ID Q9QZL0
• Background: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 (1-3). RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-kappaB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues (2,3).
• Synonyms: RIP-3; RIP3