GAPDH Goat Polyclonal Antibody
$565.00
2 Weeks*
Specifications
| Product Data | |
| Recommended Dilution | ELISA:1:128000.WB:0.01-0.5µg/ml.IHC: 5µg/ml. IHC: 5µg/ml. |
|---|---|
| Reactivity | Human, Mouse, Rat (Expected from sequence similarity: Dog) |
| Antibody Host | Goat |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2. |
| Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Concentration | 0.5 mg/ml |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | glyceraldehyde-3-phosphate dehydrogenase |
| Database Link | |
| Background | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. provided by RefSeq |
| Synonyms | G3PD; GAPD; HEL-S-162eP |
| Note | In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed |
| Reference Data | |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Loss of UBP1 drives oxaliplatin resistance through a targetable dependency on translation initiation
Abohawya, Schmäche, Dietzel et al
bioRxiv (2026)
Abohawya, Schmäche, Dietzel et al
bioRxiv (2026)
AI Summary: In this study, the OriGene GAPDH antibody (TA302944) was used as a loading control in Western blot experiments to assess protein expression changes associated with oxaliplatin resistance in gastric cancer organoids.
Snippet: , phospho-4E-BP1 (S65;#9451, CST, 1:1000), GAPDH (TA302944, Origene, 1:10000), or β-tubulin (1:1000, Santa Cruz Biotechnology, sc-166729). The next day, membranes were washed three times for 10 min each in 5% milk PBS-T and then incubated for 40 min at room temperature with secondary antibodies (IRDye 800CW and IRDye 680LT, both LI-COR Odyssey, 1:13000)
RNAi-Mediated Screen of Primary AML Cells Nominates MDM4 as a Therapeutic Target in NK-AML with DNMT3A Mutations
Sidorova, Sayed, Paszkowski-Rogacz et al
Cells (2022) 11 (5) DOI: 10.3390/cells11050854
Sidorova, Sayed, Paszkowski-Rogacz et al
Cells (2022) 11 (5) DOI: 10.3390/cells11050854
Cited by 7
AI Summary: OriGene’s anti-GAPDH antibody (Cat.No. TA302944) was used as a loading control in Western blot experiments to assess protein expression in the study.
Snippet: (PBST, 0.1%) for 30 min at room temperature prior to overnight incubation with the primary anti-MDM4 and anti-GAPDH antibodies (Proteintech, Rosemont, IL, USA;Cat.No. 17914-1-AP, 500× dilution and Origene, Rockville, MD, USA;Cat.No. TA302944, 1000× dilution, respectively) at 4 °C, diluted in 5% milk in PBST. The membrane was washed 3 times for 10 min in PBST and incubated with secondary anti
Grainyhead-like 2 Reverses the Metabolic Changes Induced by the Oncogenic Epithelial-Mesenchymal Transition: Effects on Anoikis
Farris, Pifer, Zheng et al
Mol. Cancer Res. (2016) 14 (6), 528-38 DOI: 10.1158/1541-7786.MCR-16-0050
Farris, Pifer, Zheng et al
Mol. Cancer Res. (2016) 14 (6), 528-38 DOI: 10.1158/1541-7786.MCR-16-0050
AI Summary: In this study, the GAPDH antibody from OriGene was used as a loading control in immunoblot experiments to assess protein expression changes related to GRHL2’s effects on cellular metabolism and anoikis sensitivity.
Snippet: GLUD1, rb (Abcam 168352), α-tubulin, Rb (Cell Signaling), Fibronectin, ms (BD Biosciences), GAPDH, ms (Origene), β-actin, ms (Thermo-pierce), SOD2, Rb (Cell Signalin g), HIF1α, ms (BD Biosciences), p110, ms (BD Biosciences), TOMM20, ms (BD Biosciences), VDAC1, Rb (Cell Signaling)
Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways
Herzog, Rid, Wagner et al
BMC Res Notes (2015) 8 (1), 785 DOI: 10.1186/s13104-015-1783-7
Herzog, Rid, Wagner et al
BMC Res Notes (2015) 8 (1), 785 DOI: 10.1186/s13104-015-1783-7
Cited by 5
AI Summary: In this study, OriGene's GAPDH antibody (TA302944-100) was used as a control in qRT-PCR experiments to validate differential gene expression in an epidermolysis bullosa simplex Dowling-Meara keratinocyte cell line.
Snippet: Santa Cruz Biotechnology, Inc.;Cytokeratin 19 antibody, 11-120-C020, mouse monoclonal, EXBIO Praha as;SOX2 antibody, D1951-1NB-1-1R1M1M3/1C16_110120, mouse monoclonal, ABMART;GAPDH antibody, TA302944-100, goat polyclonal, OriGene;antibodies ... Cite SaveSaving
Pseudosyndactyly - an inflammatory and fibrotic wound healing disorder in recessive dystrophic epidermolysis bullosa
Boltzmann, Gruber, Klausegger et al
J Dtsch Dermatol Ges (2015) 13 (12), 1257-66 DOI: 10.1111/ddg.12839
Boltzmann, Gruber, Klausegger et al
J Dtsch Dermatol Ges (2015) 13 (12), 1257-66 DOI: 10.1111/ddg.12839
Cited by 31
AI Summary: In this study, the polyclonal goat anti-GAPDH antibody from OriGene Technologies was used as a loading control in immunoblot experiments to assess protein expression levels in tissue samples from RDEB patients and healthy donors.
Snippet: α-SMA (1 : 1 000, Sigma-Aldrich), monoclonal mouse anti-TN-C (1 : 200, Santa Cruz Biotechnology Inc., Santa Cruz, CA), monoclonal rat anti-TGF-β1 (1 : 1 000–1 : 2 500, BD Biosciences, Franklin Lakes, NJ), polyclonal goat anti-GAPDH (1 : 2 500, Origene Technologies ... Cite SaveSaving
Showing 1-5 of 5 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
