Prohibitin (PHB) Rabbit Polyclonal Antibody

CAT#: TA302261

Rabbit Polyclonal Antibody against PHB (N-term)

Product Images

PHB1 Antibody (Human N-term) (Cat. #TA302261) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow).

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100, FC: 1:10~50
• Reactivities: Human (Predicted: Bovine, Chicken)
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29804 Da
• Gene Name: prohibitin
• Database Link:
  NP_002625
  Entrez Gene 5245 Human
  UniProt ID P35232
• Background: Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.
• Synonyms: HEL-215; HEL-S-54e; PHB1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors