MAP1LC3A Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB |
|---|---|
| Recommended Dilution | Chromatin Immunoprecipitation (ChIP), Immunoprecipitation: 20 ug / 500 ug of lysate, Immunohistochemistry: 1:200-1:400, Simple Western: 1:50, Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence: 1:100-1:300, Immunohistochemistry-Paraffin: 1:200-1:400, Flow Cytometry, ELISA, Immunohistochemistry Whole-Mount, Immunoblotting, Immunohistochemistry-Frozen, Southern Blot, Western Blot: 2.0 ug/ml |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). |
| Buffer | PBS, 0.02% Sodium Azide |
| Purification | Affinity purified |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | microtubule associated protein 1 light chain 3 alpha |
| Database Link | |
| Background | Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells. |
| Synonyms | 1BLC3; ATG8F; LC3B; MAP1A; MAP1LC3B-a |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
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| FAQs |
| SDS |
Citations
Anthracycline cardiotoxicity is exacerbated by global p38β genetic ablation in a sexually dimorphic manner but unaltered by cardiomyocyte-specific p38α loss
George, Kiss, Trampel et al
Am J Physiol Heart Circ Physiol (2023) 325 (5), H983-H997 DOI: 10.1152/ajpheart.00458.2023
George, Kiss, Trampel et al
Am J Physiol Heart Circ Physiol (2023) 325 (5), H983-H997 DOI: 10.1152/ajpheart.00458.2023
Cited by 8
AI Summary: OriGene's LC3 antibody (TA301542) was used in this study to assess autophagy levels in cardiac tissue, contributing to the investigation of molecular mechanisms underlying doxorubicin-induced cardiotoxicity.
Snippet: Severe cardiotoxic effects limit the efficacy of doxorubicin (DOX) as a chemotherapeutic agent. Activation of intracellular stress signaling networks, including p38 mitogen- Save;phospho-mTOR Ser2488 (Cell Signaling, 208 #5535, 1:500), cleaved caspase 9 (Cell Signaling Technologies, #9509, 1:1000), LC3 (Origene, 209 #TA301542, 1:500), and α-tubulin (Abcam, #4074, 1:1,000). …
p38δ genetic ablation protects female mice from anthracycline cardiotoxicity
George, Kiss, Obaid et al
Am J Physiol Heart Circ Physiol (2020) 319 (4), H775-H786 DOI: 10.1152/ajpheart.00415.2020
George, Kiss, Obaid et al
Am J Physiol Heart Circ Physiol (2020) 319 (4), H775-H786 DOI: 10.1152/ajpheart.00415.2020
Cited by 24
AI Summary: In this study, OriGene products—including the anti-LC3B antibody (Cat# 100713) and anti-GAPDH antibody (Cat# TA802519)—were used for immunoblotting to assess autophagy and protein expression levels, thereby contributing to the analysis of molecular mechanisms underlying cardioprotection in p38δ-deficient female mice.
p38delta genetic ablation protects female mice from anthracycline cardiotoxicity
George, Kiss, Obaid et al
bioRxiv (2020)
George, Kiss, Obaid et al
bioRxiv (2020)
Snippet: phospho-mTOR Ser2488 (Cell Signaling, 5535), Akt (Cell Signaling, 4691), phospho-Akt Th308 (Cell Signaling, 13038), DEPTOR (Novus Biologicals, NBP1-4967), LC3 (Origene, TA301542) and GAPDH (Abcam, ab9484)
Heme accumulation in endothelial cells impairs angiogenesis by triggering paraptosis
Petrillo, Chiabrando, Genova et al
Cell Death Differ. (2017)
Petrillo, Chiabrando, Genova et al
Cell Death Differ. (2017)
AI Summary: OriGene’s rabbit polyclonal antibody against LC3 (TA301542) was used in this study to detect the conversion of LC3-I to LC3-II, helping to assess autophagic processes in endothelial cells with altered heme homeostasis.
Snippet: Rabbit polyclonal antibody against LC3 (TA301542, Origene) was used to detect conversion of LC3-I (cytosolic) to LC3-II (autophagic vacuole membrane bound)
Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism
Russ, Boyd, McCoy et al
Biogerontology (2015)
Russ, Boyd, McCoy et al
Biogerontology (2015)
Cited by 34
Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats
Garvey, Russ, Skelding et al
Physiol Rep (2015) 3 (2) DOI: 10.14814/phy2.12319
Garvey, Russ, Skelding et al
Physiol Rep (2015) 3 (2) DOI: 10.14814/phy2.12319
Cited by 33
AI Summary: In this study, the OriGene LC3B antibody (#TA301542) was used to assess autophagy-related protein expression in skeletal muscle, contributing to the finding that voluntary running wheel activity increased autophagy markers in late middle-aged rats.
Snippet: …Antibodies against protein kinase, AMP‐activated, alpha 1 catalytic subunit (AMPK, #ab110036), phospho‐AMPK (Thr172, #ab72845), phosphorylase, glycogen, muscle (PYGM, #ab88078), and vascular endothelial growth factor A (VEGFA, #ab1316) were purchased from Abcam (Cambridge, MA). The antibody against microtubule‐associated protein 1 light chain 3 beta (LC3B, #TA301542) was purchased from OriGene (Rockville, MD). …
Showing 1-6 of 6 papers.
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