HA Rabbit Polyclonal Antibody

CAT#: TA160089

Rabbit Polyclonal Anti-Seasonal H1N1 Hemagglutinin Antibody

Product Images

Western blot analysis of Hemaggutinin using recombinant seasonal Hemagglutinin (lanes A & B) and swine-origin Hemagglutinin (lanes C & D) with anti-seasonal Hemagglutinin antibody (5235) at 2 ug/mL (lanes A & C) and anti-swine-origin Hemagglut

• 

  ELISA results using Seasonal H1N1 Hemagglutinin antibody at 1 ug/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1 ug/mL
• Reactivities: Influenza A Virus
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Hemagglutinin antibody was raised against a synthetic peptide from the Hemagglutinin protein. The peptide sequence is unique from the peptide sequence for product 5231 and 5239. This antibody is a cognate pair with antibody 5237.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 62 kDa; Observed: 68 kDa
• Background: Seasonal H1N1 Hemagglutinin Antibody: Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.