IFNG Rabbit Polyclonal Antibody

CAT#: SP2138A

IFNG rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: ELISA.
  Functional Assays: 10-50 µg/ml.
  Western Blot: This antibody detects a band of approximately 18 kDa.
  
• Reactivities: Chicken
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant Chicken Interferon Gamma
• Specificity: This antibody recognizes Chicken Interferon gamma (chIFN-gamma).
  This antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFNg-amma from that of chIFN-alpha and chIFN-beta.
• Formulation: PBS
  State: Azide Free
  State: Liquid purified IgG fraction
  Stabilizer: None
  Preservative: None
• Concentration: lot specific
• Purification: Affinity Chromatography on protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interferon, gamma
• Database Link:
  Entrez Gene 396054 Chicken
  UniProt ID P49708
• Background: Original studies investigating the properties of chicken Interferon (chIFN), revealed properties similar to mammalian IFN type I (IFNI), and it has since become apparent that there are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma. chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFNgamma. Several independent studies have since supported the existence of this chicken homolog, by the cloning of IFN-gamma cDNA from activated chicken T cells and demonstrating that recombinant chicken IFNgamma strongly upregulates the expression of MHC class II and iNOS genes.
• Synonyms: IFN-gamma, gamma IFN