Biotin Goat Polyclonal Antibody

CAT#: R1615AP

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Biotin goat polyclonal antibody, AP

Product Datasheet for 'R1615AP'

USD 760.00

3 Days

    • 1 mg

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Product Data
Applications ELISA, IHC, WB
Recommended Dilution Suitable for Immunoblotting (Western or dot blot, 1/500-1/2,500), ELISA (1/3,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Host Goat
Clonality Polyclonal
Immunogen Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Label: AP
State: Liquid (sterile filtered) purified IgG fraction
Stabilizer: 10 mg/ml BSA (Ig and Protease free)
Preservative: 0.09% (w/v) Sodium Azide
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity Chromatography
Conjugation AP
Storage Store undiluted at 2-8°C.
Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Stability Shelf life: one year from despatch.
Background Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms Vitamin B7, Vitamin H
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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