DYKDDDDK Epitope Tag Rabbit Polyclonal Antibody
$690.00
3 Days*
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:2,000 - 1:10,000 IHC: User Optimized ELISA: 1:20,000 - 1:40,000 |
| Reactivity | FLAG-Tag |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG™ conjugated proteins. |
| Specificity | This affinity purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.09 - lot specific |
| Purification | Affinity purification of this polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Background | Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. |
| Synonyms | rabbit antibody for the detection of FLAG™ conjugated proteins, rabbit anti DYKDDDDK |
| Note | This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGä containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. Rockland Immunochemical's polyclonal antibody to detect FLAG™ conjugated proteins binds FLAG™ containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. |
| Reference Data | |
Reviews
Documents
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Citations
Demonstration of Co-Infection and Trans-Encapsidation of Viral RNA In Vitro Using Epitope-Tagged Foot-and-Mouth Disease Viruses
Childs, Juleff, Moffat et al
Viruses (2021) 13 (12) DOI: 10.3390/v13122433
Childs, Juleff, Moffat et al
Viruses (2021) 13 (12) DOI: 10.3390/v13122433
Cited by 6
AI Summary: OriGene’s rabbit anti-FLAG antibody (R1180) was used in this study to detect FLAG-tagged VP1 proteins via immunofluorescence and flow cytometry, enabling the identification and analysis of co-infected cells with epitope-tagged foot-and-mouth disease viruses.
Snippet: Cells were then incubated with mouse anti-HA (Sigma H3663) and rabbit anti-FLAG (OriGene technologies R1180) antibodies in FACS diluent for 30 min, then washed three times in FACS diluent before labelling with goat anti-mouse IgG1-AF 488 and goat anti- Save Cached;hen incubated with mouse anti-HA (Sigma H3663) and rabbit anti-FLAG (OriGene technologies R1180) antibodies in FACS diluent for 30 min, then washed three times in FACS diluent before
Showing 1-1 of 1 papers.
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