wecC Goat Polyclonal Antibody

CAT#: R1155

wecC goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions. This product has been assayed against 1.0 ug of N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product:
• Reactivities: Escherichia coli
• Host: Goat
• Clonality: Polyclonal
• Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant].
• Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant].
  Cross reactivity against N-Acyl Mannosamine-1-Dehydrogenase from other sources is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent)
• Concentration: lot specific
• Purification: Multistep process.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P27829
• Background: N acylmannosamine 1 dehydrogenase belongs to the short chain dehydrogenases/ reductases family (SDR). It acts on acetyl D mannosamine and glycolyl D mannosamine.
• Synonyms: wecC, rffD, b3787, JW5599