Urease Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000 IP: 1:100 |
| Reactivity | Jack Bean |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Urease Jack Bean |
| Specificity | Anti-Urease (Jack Bean) (Rabbit) Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Urease Jack Bean. Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined. |
| Buffer | 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL |
| Concentration | 90 mg/mL - lot specific |
| Purification | Prepared from monospecific antiserum by a Delipidation and Defibrination |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research. |
| Synonyms | rabbit anti-Anti-Urease Antibody, Urease, Urea amidohydrolase |
| Note | Anti-Urease Antibody has been assayed against 1.0 ug of Urease Jack Bean in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG L - Cat# H (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Urease Antibody. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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