KTI3 Rabbit Polyclonal Antibody

CAT#: R1115PS

KTI3 rabbit polyclonal antibody, Ig Fraction


USD 662.00

3 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, IP, WB
Recommended Dilution Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
ELISA
: 1/5,000-1/20,000.
This product has been assayed against 1.0 µg of Trypsin inhibitor [soy bean] in a standard ELISA using peroxidase conjugated affinity purified anti-rabbit IgG (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/12,000 of the reconstitution concentration is suggested.
Reactivities Soy Bean
Host Rabbit
Clonality Polyclonal
Immunogen Trypsin inhibitor from soy bean
Specificity This antibody detects soy bean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [soy bean].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2
State: Ig Fraction
State: Lyophilized IgG fraction
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water or equivalent.
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-trypsin inhibitor antibody is ideal for investigators involved in cell signaling, biochemistry and signal transduction research.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.