KTI3 Rabbit Polyclonal Antibody
|Recommended Dilution||Western blot: 1/500-1/3,000.
This product has been assayed against 1.0 µg of Trypsin inhibitor [lima bean] in a standard ELISA using peroxidase conjugated affinity purified anti-rabbit IgG (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/15,000 of the reconstitution concentration is suggested.
|Immunogen||Trypsin inhibitor from lima bean|
|Specificity||This antibody detects lima bean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [lima bean].
|Formulation||0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2
State: Ig Fraction
State: Lyophilized IgG fraction
Preservative: 0.01% (w/v) Sodium azide
|Reconstitution Method||Restore with 0.1 ml of deionized water or equivalent.|
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Purification||Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.