KTI3 Rabbit Polyclonal Antibody
KTI3 rabbit polyclonal antibody, HRP
|Recommended Dilution||Western blot: 1/1,000-1/5,000.
Antibody has been assayed against 1.0 µg of Trypsin inhibitor [lima bean] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
|Immunogen||Trypsin inhibitor from lima bean|
|Specificity||This antibody detects lima bean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [lima bean].
|Formulation||0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2
State: Lyophilized IgG fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
|Reconstitution Method||Restore with 0.1 ml of deionized water or equivalent.|
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Purification||Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|