KTI3 Rabbit Polyclonal Antibody
KTI3 rabbit polyclonal antibody, Biotin
|Recommended Dilution||Western blot: 1/500-1/3,000.
This antibody has been assayed against 1.0 µg of Trypsin inhibitor in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/6,000 to 1/26,000 of the reconstitution concentration is suggested.
|Immunogen||Trypsin inhibitor from lima bean|
|Specificity||This antibody detects lima bean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [ lima bean].
|Formulation||0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2
State: Lyophilized IgG fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Sodium azide
|Reconstitution Method||Restore with 0.1 ml of deionized water or equivalent.|
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Purification||Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer|