KTI3 Rabbit Polyclonal Antibody
|Applications||ELISA, IP, WB|
|Recommended Dilution||Suitable for Immunoblotting (Western or Dot blot), ELISA and most Immunological methods requiring high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Trypsin Inhibitor [Lima Bean] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product.
|Immunogen||Trypsin Inhibitor [Lima Bean].|
|Specificity||Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Trypsin Inhibitor [Lima Bean].
Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.
|Formulation||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
State: Lyophilized purified Ig fraction.
|Reconstitution Method||Restore with 2.0 ml of deionized water (or equivalent).|
|Concentration||85.0 mg/ml (by Refractometry)|
|Purification||Prepared from monospecific antiserum by a delipidation and defibrination.|
|Storage||Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
|Stability||Shelf life: One year from despatch.|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.