SOD1 Rabbit Polyclonal Antibody

CAT#: R1111HRPS

SOD1 rabbit polyclonal antibody, HRP, Purified

Product Images

Western blot of HRP conjugated Superoxide Dismutase antibody. Lane 1: Superoxide Dismutase load: 50 ng per lane. Primary antibody: HRP conjugated Superoxide Dismutase antibody at 1/1,000 overnight at 4°C. Secondary antibody: none; HRP substrate. Predicted/observed size: 15.6 kDa, 15.6 kDa.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/5,000-1/20,000.
  Western blot: 1/500-1/2,000.
  This product has been assayed against 1.0 µg of Superoxide Dismutase [bovine erythrocytes] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/2,000 of the reconstitution concentration is suggested.
• Reactivities: Bovine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Superoxide Dismutase from Bovine erythrocytes
• Specificity: This antibody detects bovine Superoxide Dismutase. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Superoxide Dismutase [bovine erythrocytes].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamycin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 281495 Bovine
  UniProt ID P00442
• Synonyms: SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1