Horseradish Peroxidase / HRP Rabbit Polyclonal Antibody

CAT#: R1107B

Horseradish Peroxidase / HRP rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/20,000-1/100,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Reactivities: Horsereddish
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peroxidase from Horseradish.
• Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish].
  Cross reactivity against Peroxidase from other sources is unknown.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.