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Horseradish Peroxidase / HRP Rabbit Polyclonal Antibody

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SKU
R1107B
Anti-Peroxidase (Horseradish) (RABBIT) Antibody Biotin Conjugated, 10 mg
Size:
10 mg
Conjugation:
Unconjugated
  $4,030.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivity Peroxidase
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Peroxidase Horseradish
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Peroxidase Horseradish.  Cross reactivity against Peroxidase from other sources is unknown.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 10.0 mg/mL - lot specific
Purification Multi-step process
Conjugation Unconjugated
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Stability Expiration date is one (1) year from date of receipt.
Shipping Ambient
Background Peroxidase described as the removal of hydrogen peroxide, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, a response to environmental stresses such as wounding, a and pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Synonyms rabbit anti-Peroxidase Antibody biotin conjugation, rabbit anti-HRP antibody biotin conjugate, horseradish antibody biotin conjugated, rabbit anti Peroxidase biotin, rabbit anti HRP biotin
Note Anti-Peroxidase Antibody Biotin Conjugated has been tested by dot blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Reference Data
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Documents
Resources

file-alt Citations

NF‑κB/IκBα signaling pathways are essential for resistance to heat stress‑induced ROS production in pulmonary microvascular endothelial cells
Xie, Huang, Cai et al
Mol Med Rep (2021) 24 (5) DOI: 10.3892/mmr.2021.12454
Cited by 17
AI Summary: OriGene's HRP-conjugated anti-rabbit IgG antibody (cat. no. ZB-2301) was used for western blot detection of target proteins, enabling the analysis of NF-κB/IκBα signaling pathways in heat stress-induced pulmonary microvascular endothelial cells.
Snippet: no. 5174;1:1,000;Cell Signaling Technology, Inc.) overnight at 4°C. Following primary incubation, membranes were incubated with HRP-conjugated anti-rabbit IgG antibody (cat. no. ZB-2301;1:5,000;OriGene Technologies, Inc.) at room temperature for 1 h. Protein bands were visualized using ECL reagents (Pierce;Thermo Fisher Scientific, Inc.) with GAPDH and Lamin B as the internal control. Image;- n...
WB
MicroRNA-144 targets APP to regulate AML1/ETO+ leukemia cell migration via the p-ERK/c-Myc/MMP-2 pathway
Jiang, Meng, Yu et al
Oncol Lett (2019) 18 (2), 2034-2042 DOI: 10.3892/ol.2019.10477
AI Summary: OriGene's HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were used in western blot experiments to detect protein expression levels, contributing to the analysis of signaling pathways in AML1/ETO+ leukemia cell migration.
Snippet: … cs-13594;dilution of 1:1,000;Santa Cruz Biotechnology Inc.), MMP-9 (catalog no. cs-21733;dilution of 1:500;Santa Cruz Biotechnology Inc.), p-ERK (catalog no. …
WB
Showing 1-2 of 2 papers.
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