Horseradish Peroxidase / HRP Rabbit Polyclonal Antibody
$735.00
3 Days*
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivity | Peroxidase |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Anti-Peroxidase antibody was produced by repeated immunization of rabbits with peroxidase isolated from horseradish. |
| Specificity | Anti-Peroxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Peroxidase Horseradish. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined. |
| Buffer | See application note. Stabilizer: None Preservative: None |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL |
| Concentration | 90 mg/mL - lot specific |
| Purification | Prepared from monospecific antiserum by a Delipidation and Defibrination |
| Conjugation | Unconjugated |
| Storage | Store anti-HRP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Background | Anti-Peroxidase antibody is a valuable tool for most common immunoassays including immunoprecipitation (IP), ELISA, western blotting (WB) and immunohistochemistry (IHC). |
| Synonyms | rabbit anti-HRP, Peroxidase antibody, anti-HRP, Peroxidase antibody raised in rabbit, Rb-anti-HRP, Rb-anti-Peroxidase, Horseradish Peroxidase |
| Note | Anti-Peroxidase antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. Note the buffer for this anti-peroxidase antibody is 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.6 |
| Reference Data | |
Reviews
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Citations
Targeting ANXA7/LAMP5-mTOR axis attenuates spinal cord injury by inhibiting neuronal apoptosis via enhancing autophagy in mice
Li, Chen, Zhao et al
Cell Death Discov (2023) 9 (1), 309 DOI: 10.1038/s41420-023-01612-w
Li, Chen, Zhao et al
Cell Death Discov (2023) 9 (1), 309 DOI: 10.1038/s41420-023-01612-w
Cited by 23
AI Summary: OriGene products contributed to this study by providing the ANXA7 overexpression lentivirus (OriGene, Cat# RC220215L2) and LAMP5 overexpression plasmid (OriGene, Cat# MR221446), which were used to investigate the functional roles of ANXA7 and LAMP5 in neuronal autophagy and apoptosis following spinal cord injury in mice.
Alcoholic Liver Disease Is Associated with Elevated Plasma Levels of Novel Advanced Glycation End-Products: A Preliminary Study
Litwinowicz, Waszczuk, Kuzan et al
Nutrients (2022) 14 (24) DOI: 10.3390/nu14245266
Litwinowicz, Waszczuk, Kuzan et al
Nutrients (2022) 14 (24) DOI: 10.3390/nu14245266
Cited by 9
AI Summary: OriGene’s anti-RAGE antibody (Catalog # TA809082) was used in this study to detect RAGE protein expression, contributing to the analysis of advanced glycation end-product (AGE) involvement in alcoholic liver disease.
miR-let-7c-3p targeting on Egr-1 contributes to the committed differentiation of leukemia cells into monocyte/macrophages
Qi, Wang, Zhao et al
Oncol Lett (2022) 24 (2), 273 DOI: 10.3892/ol.2022.13393
Qi, Wang, Zhao et al
Oncol Lett (2022) 24 (2), 273 DOI: 10.3892/ol.2022.13393
Cited by 3
AI Summary: In this study, OriGene products including EGR1 siRNA (SR300359) and EGR1 expression plasmid (RC206307) were used to modulate Egr-1 gene expression, enabling the authors to investigate the role of the miR-let-7c-3p/Egr-1 signaling axis in the differentiation of leukemia cells into monocytes/macrophages.
The X box binding protein 1/C/EBP homologous protein pathway induces apoptosis of endothelial cells under hyperglycemia
Tang, Zheng, Li et al
Exp Ther Med (2022) 24 (1), 454 DOI: 10.3892/etm.2022.11381
Tang, Zheng, Li et al
Exp Ther Med (2022) 24 (1), 454 DOI: 10.3892/etm.2022.11381
AI Summary: OriGene products contributed to this study through the use of OriGene siRNAs targeting XBP1 (si-XBP1, Cat# SR321382) and CHOP (si-CHOP, Cat# SR304274), which were employed to specifically downregulate these genes and investigate their roles in hyperglycemia-induced endothelial cell apoptosis.
Monensin suppresses cell proliferation and invasion in ovarian cancer by enhancing MEK1 SUMOylation
Yao, Wang, Zhou et al
Exp Ther Med (2021) 22 (6), 1390 DOI: 10.3892/etm.2021.10826
Yao, Wang, Zhou et al
Exp Ther Med (2021) 22 (6), 1390 DOI: 10.3892/etm.2021.10826
Cited by 23
AI Summary: OriGene products, specifically the MEK1 plasmid (RC200176) and MEK1-specific shRNA (TL312431), were used in this study to manipulate MEK1 expression and investigate the role of MEK1 SUMOylation in the anti-cancer effects of monensin on ovarian cancer cells.
Interference with the expression of S1PR1 or STAT3 attenuates valvular damage due to rheumatic heart disease
Xian, Chen, Wu et al
Int J Mol Med (2021) 48 (3) DOI: 10.3892/ijmm.2021.5012
Xian, Chen, Wu et al
Int J Mol Med (2021) 48 (3) DOI: 10.3892/ijmm.2021.5012
Cited by 15
AI Summary: OriGene’s anti-rabbit (cat. no. PV-6001) and anti-mouse (cat. no. PV-6002) secondary antibodies were used in immunohistochemistry experiments to detect protein expression in valvular tissues, supporting the study’s investigation of S1PR1 and STAT3 roles in rheumatic heart disease.
Snippet: 1:10;cat. no. PV-6001;OriGene Technologies, Inc.) or anti-mouse secondary antibody (1:10;cat. no. PV-6002;OriGene Technologies, Inc.) were incubated with the sections for 30 min at room temperature. After enhanced colour;- 1:10;cat. no. PV‑6001;OriGene Technologies, Inc.) or anti‑mouse secondary antibody (1:10;cat. no. PV‑6002
Showing 1-6 of 28 papers.
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