pep1 Rabbit Polyclonal Antibody

CAT#: R1105

pep1 rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/5,000 - 1/20,000.
  Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
  ELISA: 1/30,000 - 1/160,000.
  This product has been assayed against 1.0 µg of Phospho-enol-pyruvate carboxylase [maize leaves] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/30,000 to 1/160,000 of the reconstitution concentration is suggested.
• Reactivities: Maize
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Phospho-enol-pyruvate Carboxylase [Maize].
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Phospho-enol-pyruvate Carboxylase [Maize Leaves].
  Cross reactivity against Phospho-enol-pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P04711
• Synonyms: PPC, PEPC1, PEPC 1, PEPCase 1, PEP carboxylase