Penicillinase Rabbit Polyclonal Antibody

CAT#: R1104BS

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Penicillinase rabbit polyclonal antibody, Biotin, Purified



USD 540.00


Availability*
3 Days

Size
    • 100 ug


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Specifications

Product Data
Applications ELISA, IP, WB
Recommended Dilution ELISA: 1/5,000-1/20,000.
This product has been assayed against 1.0 µg of Penicillinase [E.clocae] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
Reactivity Enterobacter
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Penicillinase from Enterobacter cloacae
Specificity This antibody detects Penicillinase [E. clocae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Penicillinase [E. clocae].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: Biotin
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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