Penicillinase Rabbit Polyclonal Antibody
Penicillinase rabbit polyclonal antibody, Biotin, Purified
|Applications||ELISA, IP, WB|
|Recommended Dilution||ELISA: 1/5,000-1/20,000.
This product has been assayed against 1.0 µg of Penicillinase [E.clocae] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Western blot: 1/500-1/5,000.
|Immunogen||Penicillinase from Enterobacter cloacae|
|Specificity||This antibody detects Penicillinase [E. clocae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Penicillinase [E. clocae].|
|Formulation||0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Sodium azide
|Reconstitution Method||Restore with 0.1 ml of deionized water (or equivalent).|
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Purification||Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|