Penicillinase Rabbit Polyclonal Antibody

CAT#: R1104

Penicillinase rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and most Immunological methods requiring high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Penicillinase [Enterobacter cloacae] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) (R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1:6,000 to 1:26,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Enterobacter
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Penicillinase [Enterobacter cloacae].
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Penicillinase [Enterobacter cloacae].
  Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01% (w/v) sodium azide as preservative.
  State: Serum
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Prepared from monospecific antiserum by a delipidation and defibrination.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.